How does the Omni Pro 12 work?
Omni Pro 12
The Omni Pro 12™ is an automated, high-throughput live-cell analysis platform designed for continuous multiwell imaging inside the incubator. Enhance your throughput and workflow through the unparalleled ease-of-use, flexibility, and multiplate capacity of the Omni Pro 12. The multifaceted device can shed light on cell viability, morphology, and function via brightfield and fluorescence live-cell imaging, combined with reliable and powerful data analysis.
Key Features
The automated, multi-plate platform
>> Support multiple users and applications
The Omni Pro 12 accommodates up to 12 microwell plates at a time and supports an array of applications and analyses.
>> Assay your cells in brightfield and fluorescence
From label-free cell monitoring to fluorescence-based assays, multiple imaging modes allow the device to visualize complex biological changes.
>> Track every moment, from your incubator
The Omni Pro 12 fits inside an incubator, automatically capturing images of your cells as they grow in their optimal environment.
>> Place your plate and walk away
The automated robotic arm works around the clock by placing microplates on the stationary sample stage. The software also allows you to monitor your cells and perform data analysis from your desktop.
>> Get started quickly
A short training will get you up and running with the Omni Pro 12. The platform is easy to use and requires no maintenance or calibration.
Multiplex your assay: Maestro + Omni
>> Platform cross-compatibility lets you view live-cell imaging alongside real-time, functional data to enhance your analysis
>> Monitor electrode coverage and cell morphology to ensure consistent, quality results
>> Experience no-hassle tracking—the Axion Portal automatically links data via barcode
Overview
Omni Pro 12 overview
The Omni Pro 12 platform is designed to simplify and accelerate the acquisition of complex biological data. Using brightfield and fluorescence imaging combined with advanced software tools, the Omni Pro 12 can help you to examine cell health and function non-invasively and in real time.
Software Modules
The Omni Pro 12 platform has six different software modules available to meet your assay needs:
Confluency Module – Know exactly when to passage your cells or track cell growth and death in your experiments with brightfield confluency.
Clonogenic Assay Module – Monitor and quantify cell proliferation and colony formation to study the effects of cytotoxic and immunotherapeutic agents.
Fluorescence Module – Add fluorescent assay capabilities with fluorescence confluency, intensity, and object detection.
iPSC Module – For monitoring the growth of pluripotent stem cells in a feeder-free culture environment.
Organoid Analysis Module – Identify, track, and analyze organoids across an array of culture vessels using an advanced machine-learning algorithm.
Scratch Assay Module – Investigate the progression of wound closure over time.
Cross-compatible Platforms
View data collected with your Omni™ within the software of these compatible platforms:
Maestro MEA™ – See whole-plate images of your cultures next to real-time activity measurements. Confirm electrode coverage and culture health with just a few clicks. Compatible with the Maestro Pro™ and Edge™ systems.
Maestro Z™ – Complement your impedance assays with whole-plate images of your cultures. Monitor electrode coverage and culture health over time with just a few clicks. Compatible with the Maestro ZHT™, TrayZ™, and Z systems.
FAQ:
Omni Pro 12 is a high-throughput live-cell imaging platform, consisting of an Omni FL system with integrated 12-plate storage and handling. Samples are illuminated using an LED and recorded with a moving camera positioned below the sample stage. During brightfield acquisition, the camera scans the sample stage and acquires a series of sequential images. One complete brightfield scan generates around 7850 snapshots. These are stitched to form an image with a surface area of 86 mm × 124 mm (3.4’’ × 4.9’’). When acquiring fluorescence images, the users can choose how many snapshots of a defined location within the well they want to record. The images are uploaded to the portal where they can be analyzed using our image analysis algorithms or 3rd party software.
What type of image analysis modules can I use?
The Omni Pro 12 is compatible with the following modules: Confluency Module, Scratch Assay Module, Clonogenic Assay Module, Organoid Analysis Module, and Fluorescent Object Count. Users always have the option to download the raw data and perform their own analysis on 3rd party software.
Can the Omni Pro 12 be used inside a cell culture incubator?
Yes, the Omni Pro 12 is designed to be used inside a cell culture incubator. The hardware and electronics can operate at 5 – 40°C and between 20 – 95% humidity.
What culture vessels are compatible with the Omni Pro 12?
Any transparent microplate (e.g. 6- to 384-well plate) is compatible with the Omni Pro 12.
Related Applications
