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Neural field potential recordings were performed using the Maestro Pro MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 768 electrodes (12.5 kHz sampling rate, 200-3000 Hz band-pass). Analysis was performed using AxIS Navigator software (Axion BioSystems).

Local field potential (LFP) recordings were performed using the Maestro Pro MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 768 electrodes (12.5 kHz sampling rate, 1-5000 Hz band-pass). Low frequency LFP components were separated from high frequency neural spikes using a digital Butterworth bandpass filter of 1-200 Hz and 200-2000Hz, respectively. Analysis was performed using AxIS Navigator software (Axion BioSystems).

Cardiac field potential recordings were performed using the Maestro Pro MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 768 electrodes (12.5 kHz sampling rate, 1-2000 Hz band-pass). Analysis was performed using AxIS Navigator software and the Cardiac Analysis Tool (Axion BioSystems).

Local extracellular action potential (LEAP) recordings were performed using the Maestro Pro MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 768 electrodes (12.5 kHz sampling rate, 0.01-2000 Hz band-pass). LEAP induction was performed by enhancing cell coupling through electrical stimulus for 10 minutes using the AxIS Navigator software (Axion BioSystems). Analysis was performed using AxIS Navigator and the Cardiac Analysis Tool software (Axion BioSystems).

Contractility was measured using the Maestro Pro MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 768 electrodes by measuring impedance at 3.125 kHz. Voltage was simultaneously recorded for cardiac field potential recordings (12.5 kHz sampling rate, 1-2000 Hz band-pass). Analysis was performed using AxIS Navigator software (Axion BioSystems).

Neural field potential recordings were performed using the Maestro Edge MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 384 electrodes (12.5 kHz sampling rate, 200-3000 Hz band-pass). Analysis was performed using AxIS Navigator software (Axion BioSystems).

Local field potential (LFP) recordings were performed using the Maestro Edge MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 384 electrodes (12.5 kHz sampling rate, 1-5000 Hz band-pass). Low frequency LFP components were separated from high frequency neural spikes using a digital Butterworth bandpass filter of 1-200 Hz and 200-2000Hz, respectively. Analysis was performed using AxIS Navigator software (Axion BioSystems).

Cardiac field potential recordings were performed using the Maestro Edge MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 384 electrodes (12.5 kHz sampling rate, 1-2000 Hz band-pass). Analysis was performed using AxIS Navigator software and the Cardiac Analysis Tool (Axion BioSystems).

Local extracellular action potential (LEAP) recordings were performed using the Maestro Edge MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 384 electrodes (12.5 kHz sampling rate, 0.01-2000 Hz band-pass). LEAP induction was performed by enhancing cell coupling through electrical stimulus for 10 minutes using the AxIS Navigator software (Axion BioSystems). Analysis was performed using AxIS Navigator and the Cardiac Analysis Tool software (Axion BioSystems).

Contractility was measured using the Maestro Edge MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 384 electrodes by measuring impedance at 3.125 kHz. Voltage was simultaneously recorded for cardiac field potential recordings (12.5 kHz sampling rate, 1-2000 Hz band-pass). Analysis was performed using AxIS Navigator software (Axion BioSystems).

Neural field potential recordings were performed using the Maestro Volt MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 384 electrodes (12.5 kHz sampling rate, 200-3000 Hz band-pass). Analysis was performed using AxIS Navigator software (Axion BioSystems).

Local field potential (LFP) recordings were performed using the Maestro Volt MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 384 electrodes (12.5 kHz sampling rate, 1-5000 Hz band-pass). Low frequency LFP components were separated from high frequency neural spikes using a digital Butterworth bandpass filter of 1-200 Hz and 200-2000Hz, respectively. Analysis was performed using AxIS Navigator software (Axion BioSystems).

Cardiac field potential recordings were performed using the Maestro Volt MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 384 electrodes (12.5 kHz sampling rate, 1-2000 Hz band-pass). Analysis was performed using AxIS Navigator software and the Cardiac Analysis Tool (Axion BioSystems).

Local extracellular action potential (LEAP) recordings were performed using the Maestro Volt MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 384 electrodes (12.5 kHz sampling rate, 0.01-2000 Hz band-pass). LEAP induction was performed by enhancing cell coupling through electrical stimulus for 10 minutes using the AxIS Navigator software (Axion BioSystems). Analysis was performed using AxIS Navigator and the Cardiac Analysis Tool software (Axion BioSystems).

Contractility was measured using the Maestro Volt MEA platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Real-time data was collected simultaneously across all 384 electrodes by measuring impedance at 3.125 kHz. Voltage was simultaneously recorded for cardiac field potential recordings (12.5 kHz sampling rate, 1-2000 Hz band-pass). Analysis was performed using AxIS Navigator software (Axion BioSystems).

Impedance measurements were performed using the Maestro Z real-time cellular impedance platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Impedance was measured every minute at 1, 10, and 41.5 kHz across all 384 electrodes. Analysis was performed using AxIS Z software (Axion BioSystems).

Impedance measurements were performed using the Maestro ZHT real-time cellular impedance platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Impedance was measured every minute at 1, 10, and 41.5 kHz across all 768 electrodes. Analysis was performed using AxIS Z software (Axion BioSystems).

Impedance measurements were performed using the Maestro TrayZ real-time cellular impedance platform (Axion BioSystems, USA) at 37 °C and 5% CO₂. Impedance was measured across all 384 electrodes of each plate every four minutes at 1, 10, and 41.5 kHz. Analysis was performed using AxIS Z software (Axion BioSystems).

Whole-plate, live-cell imaging was performed with the Omni live-cell imaging platform (Axion BioSystems, USA). Real-time high-resolution brightfield images were collected using a 10x objective inside an incubator (37°C, 5% CO₂). Analysis was performed using AxIS Vue software (Axion BioSystems, USA) with the Axion Portal (Axion BioSystems, USA).

Fluorescent imaging was performed with the Omni live-cell imaging platform (Axion BioSystems, USA). Green (ex. 452/45 nm, em. 512/23 nm) and red (ex. 561/23 nm, em. 630/90 nm) fluorescent images collected using a 10x objective inside an incubator (37°C, 5% CO₂). Analysis was performed using AxIS Vue software (Axion BioSystems, USA) with the Axion Portal (Axion BioSystems, USA).

Whole-plate, live-cell imaging was performed with the Omni Pro 12 live-cell imaging platform (Axion BioSystems, USA). Real-time high-resolution brightfield images were collected using a 10x objective inside an incubator (37°C, 5% CO₂). Analysis was performed using AxIS Vue software (Axion BioSystems, USA) with the Axion Portal (Axion BioSystems, USA).

Fluorescent imaging was performed with the Omni Pro 12 live-cell imaging platform (Axion BioSystems, USA). Green (ex. 452/45 nm, em. 512/23 nm) and red (ex. 561/23 nm, em. 630/90 nm) fluorescent images collected using a 10x objective inside an incubator (37°C, 5% CO₂). Analysis was performed using AxIS Vue software (Axion BioSystems, USA) with the Axion Portal (Axion BioSystems, USA).

Whole-plate, live-cell imaging was performed with the Lux3 live-cell imaging platform (Axion BioSystems, USA). Real-time high-resolution brightfield images were collected using a 10x objective inside an incubator (37°C, 5% CO₂). Analysis was performed using AxIS Vue software (Axion BioSystems, USA) with the Axion Portal (Axion BioSystems, USA).

Fluorescent imaging was performed with the Lux3 live-cell imaging platform (Axion BioSystems, USA). Green (ex. 452/45 nm, em. 512/23 nm) and red (ex. 561/23 nm, em. 630/90 nm) fluorescent images collected using a 10x objective inside an incubator (37°C, 5% CO₂). Analysis was performed using AxIS Vue software (Axion BioSystems, USA) with the Axion Portal (Axion BioSystems, USA).