Optical stimulation system for multiwell plates
Incorporate cutting-edge optical techniques into your in vitro research using Axion BioSystems’ Lumos. Previous in vitro optical stimulation devices provided low throughputs or limited control of light delivery, hindering the adoption of optogenetic techniques in research and development. Lumos is the first commercial multiwell optical stimulation system to offer precise control over intensity and duration to four stimulation wavelengths in each well. Designed to pair with the Maestro Pro and Edge recording platforms, the Lumos allows researchers to simultaneously direct and record functional network activity.
The Lumos advantage
- High-throughput – Control light delivery to each well of a standard 24- or 48-well microplate
- Fully configurable – Control intensity from each of 94 (24-well) or 192 (48- well) LEDs (four wavelengths per well) with microsecond precision
- Optimized light delivery – Market leading light intensities are evenly distributed across each well
- User friendly software – Design optical stimulation patterns quickly with intuitive “drag-and-drop” style building blocks
- Environmental control compatible – Regulate temperature, CO₂, and humidity for extended benchtop work
- Pairs with Axion’s Maestro Pro and Edge MEA systems – Optically control cell activity during real-time recordings of functional responses
Lumos Optical Stimulation System
The Lumos system can be used in two general ways:
- Control of cell biology – Deliver light to Axion’s Lumos OptiClear plates (24- and 48-well formats), standard-format SLAS-compatible microplates with optical specializations and clear bottom.
- Control of cell activity – Pair Lumos with Axion’s Maestro microelectrode array (MEA) platform and deliver light to Axion’s Lumos MEA plates for measurement of network activity.
Use Lumos To Control Cell Biology
Control of cellular biology on a high-throughput scale
Light-mediated control of biochemical, genetic, and cellular functions is an emerging tool in many research fields:
- Control intracellular signaling pathways – e.g. Kennedy et al. 2010
- Direct gene expression with light – e.g. Konermann et al. 2013
- Edit genes with light-induced CRISPR/Cas9 – e.g. Polstein & Gersbach 2015
- Influence growth and regeneration – e.g. Park et al. 2015
Environmental Control With ECbase
Perform extended light delivery experiments on the bench
- Temperature – Compatible with standard lab warming plates
- CO2 – Gas delivery port enables a controlled CO2 environment
- Humidity – Lumos plate humidity reservoirs reduce evaporation from wells
Lumos Opticlear Plate
Optimal light delivery and imaging
Lumos OptiClear 24 (OPT-24) and 48 (OPT-48) are Axion’s first microplates without electrodes. These plates have a high clarity bottom culture surface for microscopy and high content imaging. The specially formulated reflective white walls and lensed lid facilitate optimal light delivery within each well.
Maestro™ Pro and Edge MEA Systems
Decode life’s circuitry with MEA
The Maestro™ systems are the industry’s first high-throughput microelectrode array (MEA) systems. Record networks of electrically active cells (e.g. neurons, cardiomyocytes, and muscle cells) in a multiwell plate in real-time. Over minutes or months, gain unprecedented access to electrical network function from cultured cell populations. Perfect for characterizing newly created iPS cells, researching the behavior of diseased cells, screening for drugs to treat that disease, or examining potential toxic effects of drug candidates, the Pro and Edge systems deliver an easy-to-use, compact cellular analysis system suitable for any lab.
Use Lumos To Control Cell Activity
Control cell activity with light
Advantages of optogenetic-mediated control of cell activity include:
- Artifact-free stimulation – Light-evoked electroactivity is artifact-free
- Precise control of cell activity – Study a range of physiologically relevant states in each well
- Reduced experimental variability – Standardize cell activity to reduce variability in well-to-well and batch-to-batch activity
- Cell specific control – Enhanced neural disease modeling is possible with cell type specific control in mixed cell cultures
Lumos MEA Plates
Lumos MEA 24 (M384-tMEA-24OPT) and 48 plates (M768-tMEA48OPT) for the Maestro Pro and Edge MEA platforms combine unparalleled access to cellular electrical network information (16 recording electrodes per well) with a thin, transparent plate bottom for culture visualization and assay multiplexing. The specially formulated reflective white walls and lensed lid facilitate optimal light delivery within each well.
Revolutionizing Optical Stimulation
A first-of-its-kind on the market, Lumos revolutionizes in vitro optical approaches. Lumos provides 24 or 48 concurrent wells of stimulation and the ability to simultaneously use up to four different wavelengths of light per well at user-specified, precisely defined output patterns.
Axis Navigation - Stimulation Studio
AxIS Navigator provides intuitive, visual stimulation programming in an easily accessible plate map format. Here, each of the LEDs can be independently controlled for both intensity (1-100% max power) and duration (down to 100 microseconds). Additionally, stimulation across the plate can be set up for predefined time courses or regulated via an operator start-stop interface in the software.