Cell Proliferation

 

Visualize the Growth Visualize the Growth Potential of your Cells with Live-Cell Imaging

 

Confluence

Cell proliferation is characterized by cell growth and division, resulting in an exponential increase in total cell number and tissue growth. Various environmental factors (e.g., temperature, pH levels, chemical or infectious agents) can either promote or inhibit cell proliferation, and the study of these factors represents an important area of drug discovery and in vitro cell biology research. The intuitive Cell Confluence Module for the Omni platforms makes it easy to track and analyze cell proliferation in real time.

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Visualize the Growth Monitor and Quantify Cell Proliferation in Brightfield and Fluorescence

C6 Proliferation
FIGURE 1: Proliferation of C6 cells over a 5-day period.

Cell proliferation can be used to evaluate normal cell health, to study growth and development of cancer cells, or to measure the effectiveness of cytotoxic agents. The Cell Confluence Module for the Omni and Lux devices, tracks and quantifies cell growth automatically using image analysis.

 

Here, C6 glioma cells were imaged for 5 days (120 hours) using live-cell imaging platform. A proliferation curve was automatically generated by the Cell Confluence Module. Over the period of 5 days, the culture progresses to the exponential growth phase, until a plateau is reached at nearly 100% coverage and growth is halted.

 

 

Visualize the Growth Improve Accuracy Using Whole-Well Analysis of Cell Confluence

Cells don’t always plate evenly. Omni can image the entire plate calculating confluence across the entire well.

 

Cell Confluence Omni

 

In this example, JnHEK cells were monitored over a 48-hour period. The confluence mask(green overlay) is generated automatically to highlight the position of cells, as detected by the Cell Confluence Module.

 

 

Key Features

 

>> Assay your cells in brightfield and fluorescence – From label-free cell monitoring to fluorescence-based assays, add dynamic visual data in any experiment.

>> Maintain the optimal culture environment – The Omni and Lux platforms operate within an incubator.

>> Track every moment – Automatically capture images as your cells grow in their optimal environment.

>> Get started quickly – The live-cell analysis platforms are designed to be simple and flexible. With a host of analysis modules available, it is easy to adapt the systems to meet your needs.

 

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Question  Explore Axion's Imaging Platforms

Omni

Multiwell live-cell analysis with brightfield and fluorescent imaging from your incubator

 

Lux

Single-well live-cell analysis with brightfield and fluorescent imaging from your incubator

 

Omni Pro 12

Automated, multi-plate, live-cell analysis system