Zebrafish

Zebrafish
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Zebrafish are a great translational model of whole organ systems. Their accessibility, ease of genetic engineering, and simple behavioral screens make them useful models for many diseases. Zebrafish models are often used for toxicology and drug discovery research.

Expand on standard zebrafish assays with noninvasive electrophysiological readouts from the brain and spinal cord. Measure real-time drug interactions in live zebrafish. Axion BioSystems’ multielectrode array (MEA) assays are well-suited for studying zebrafish neurobiology.

Scale up organism electrophysiology

Measuring neural activity in living zebrafish
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Measuring neural activity in living zebrafish has always been a challenge. Consistently capture zebrafish electrophysiology simultaneously across multiple wells from live zebrafish with MEA. Multiple electrodes measure independently across the central nervous system to evaluate neural networks non-invasively and label-free. Specific regions, like the forebrain, midbrain, or hindbrain, may be targeted based on the zebrafish orientation in the well.

Zebrafish on MEA plate
Zebrafish on MEA plate

(A) Inverted microscope image of zebrafish larva in a CytoView MEA plate. (B) Neural activity map illustrating that the detected extracellular activity aligns with zebrafish orientation.

The transparent CytoView plates let you identify which electrodes correspond to which region in the spinal cord or brain. With the option to multiplex standard zebrafish imaging assays on CytoView plates after measuring activity, MEA data can easily supplement many workflows. While recording, regions of activity light up bright on the activity map in AxIS Navigator while uncovered electrodes remain dark.

 

Continuous data recorded from 3 electrodes in one well.
Well-wide raster plot demonstrates zebrafish spinal cord activity is organized into synchronous bursts.

(C) Continuous data recorded from 3 electrodes along a zebrafish spinal cord. (D) Well-wide raster plot demonstrates zebrafish spinal cord activity is organized into synchronous bursts.

AxIS Navigator lets you analyze spiking events from a single electrode, identify and sort individual neurons, or compare between multiple electrodes; allowing you to analyze localized as well as network or region-wide nervous system activity. Zebrafish electrophysiology has never been easier or more accessible than with the Maestro MEA.